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IVF

In Vitro Fertilization


IVF


I.V.F. achieves pregnancy through the fertilization of the woman's eggs (oocytes) outside of her body. The oocytes are obtained vaginally using a transvaginal sonographic procedure. The semen is processed in order to harvest the most robust sperm.

The egg and sperm are incubated together. Next day, the fertilization is evaluated. The embryos are ready for transfer at the end of 48 hours. I.V.F. is ideal in cases of tubal blockages, tubal adhesions, and selective cases of endometriosis and genital tuberculosis.

Micromanipulation/Intra Cytoplasm Sperm Injection


In this procedure, the oocyte is prepared by removing its outer coat of cells called the cumulus complex. Once laid bare, the oocyte is gently held by a holding pipette under inverted microscope.

The sperm prepared for microinjection is injected through a very fine micropipette into the cytoplasm of the oocyte. The holding pipette is 20 times thinner than the human hair, while the injection pipette is 60 times thinner than human hair. The egg itself is no bigger than the tip of a pin. The microinjected egg is returned to the incubator and checked the following day for fertilization.

Micromanipulation is ideally suited for cases of very low sperm counts or zero count (azoospermia), very little motility or complete lack of motility, and abnormal shape and size of the sperm. Its use is extended to women with advancing age, endometriosis, and unexplained infertility.



Assisted Hatching


This is the procedure in which the zona (shell) of the embryo is opened using techniques of Micromanipulation. This facilitates its hatching in the uterus and improves implantation and pregnancy rates. Assisted hatching is useful in older women.

IUI


The semen sample is processed by washing, centrifugation, and migration.

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Andrology Laboratory


The Andrology laboratory uses Semen Quality Analysis system. Key information provided include volume, pH, viscosity, morphology, number of immature cells and white blood cells, and other routine assessment. Semen quality assessment must be done in a quality-controlled laboratory, not a routine pathological laboratory.



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We at Gupte Hospital IVF centre have compassionate approach to all our patients and assure of a 24/7 service and care.

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